}#PageList1 {margin-bottom:0px} .content-outer { -webkit-box-shadow:none; box-shadow:none; } #ContactForm1 { display: none ! important; } -->

September 6, 2012

Junk DNA Turns Out To Be Extremely Important



Epigenomic Map
 
Genomics
DNA chunks that once were dismissed as “junk” turn out to play critical roles in controlling how cells, organs and other tissues behave. The discovery, considered a major medical and scientific breakthrough, has enormous implications for human health and personalized medicine.
O
ur genome is packed with at least four million gene switches that reside in bits of DNA that once were dismissed as “junk” but that turn out to play critical roles in controlling how cells, organs and other tissues behave. The discovery, considered a major medical and scientific breakthrough, has enormous implications for human health because many complex diseases appear to be caused by tiny changes in hundreds of gene switches.

Most of the DNA alterations that are tied to disease do not alter protein-coding genes, but rather the “switches” that control them. Characterizing these switches is one of many goals of the Encyclopedia of DNA Elements (ENCODE) project — a sweeping, international effort to create a compendium of all the working parts of the human genome that have not been well studied or well understood.

The function of the vast majority of the human genome has remained largely unknown, but the ENCODE project, launched in 2003, set out to change that. Composed of more than 30 participating institutions, including the Broad Institute of Harvard and MIT, the ENCODE Project Consortium has helped to ascribe potential biochemical function to a large fraction of the noncoding genome. This work has revealed elements that act like dimmer switches, subtly turning up or down a gene’s activity and influencing what parts of the genome are utilized in different kinds of cells. The team characterized and mapped out the locations of thousands of these switches and signals. More than 30 papers detailing these results appear online in Nature, Science, Genome Research, and Genome Biology this week.

“With these maps in hand, we can begin to understand why genetic variants that land in the annotated regions may predispose people to disease,” said Bradley Bernstein, a senior associate member at the Broad Institute and an associate professor of pathology at Harvard-affiliated Massachusetts General Hospital (MGH) and Harvard Medical School. Bernstein is also a principal investigator in the ENCODE Consortium. “It turns out that many of the variants that genetic researchers have tied to various diseases — lupus, Crohn’s disease, metabolic diseases, high cholesterol, and much more — sit in these regions that alter how genes are expressed in specific kinds of cells.”

Researchers from the Broad, the Massachusetts Institute of Technology (MIT), and MGH found that variants associated with autoimmune diseases such as lupus and rheumatoid arthritis sit in regions that are active only in immune cells, whereas variants tied to cholesterol and metabolic diseases sit in regions active in liver cells.

In order to generate detailed maps of the switches that lie between — and even nestled within — genes, data collection centers in the consortium amassed high-quality and comprehensive datasets detailing the function of elements in the genome. By looking across more than 140 cell types, they generated more than 1,500 datasets.

Several groups at the Broad Institute contributed to this work, including the Genome Sequencing and Analysis Program and the Epigenomics Program, which helped generate many of the datasets for the project. The term “epigenome” refers to a layer of chemical information on top of the genetic code, which helps determine when and where (and in what types of cells) genes will be active. This layer of information includes a suite of chemical changes that appear across the genetic landscape of every cell, and can differ dramatically between cell types. Researchers at the Broad and at the other ENCODE data collection centers developed ways to characterize these epigenetic “marks” across cell types.

“By bringing together computational groups from across the world and gathering all of the data generated, we can get at much more complex questions,” said Manolis Kellis, an associate member of the Broad Institute, principal investigator at the MIT Computer Science and Artificial Intelligence Lab (CSAIL), and an associate professor of computer science at MIT. Kellis is also head of the MIT Computational Biology Group and a principal investigator in the ENCODE Consortium.

The ENCODE project challenged members of the Broad’s Epigenomics Program to create new tools and methods and improve data generation. “As a data production center, we take very seriously our responsibility to only let high-quality data out the door,” said Chuck Epstein, who manages the Epigenomics Program. “We’re constantly trying to develop better ways to know that data are high quality, and that involves developing good systems to annotate and view data.”

The project also led to challenging new problems for data integration across different labs and institutions using a variety of experimental protocols. “We developed novel statistical methods and robust automated pipelines for uniform processing, quality control, reproducibility analysis, and integration of massive amounts of diverse data,” said Anshul Kundaje, who led the data coordination efforts of the consortium and is now a research scientist at the MIT Computational Biology Group. “This resulted in very high standards of data quality across the consortium as well as freely available software pipelines that we believe will serve as a valuable resource to the larger scientific community.”

With epigenomic maps in hand, researchers are now turning to the next phase of the project. They will look across more cell types — each of which contain different epigenomic instructions — and will begin looking at the wiring of these switches.

“We now have a map of the genomic locations of these switches, but we don’t have a map showing which switch controls which gene,” said Bernstein. “What turns on the switch? And when it turns on, what gene or genes get upregulated? Having a map of the way these elements are wired and connected is a critical goal.”

SOURCE  Harvard Gazette

By 33rd SquareSubscribe to 33rd Square


No comments :

Post a Comment

Thank you for your comment!

 
The Story of the Chessboard


The classic parable of how the inventor of the game of chess used his knowledge of exponential growth to trick an emperor is commonly used to explain the staggering and accelerating growth of technology. The 33rd square on the chessboards represents the first step into the second half of the chessboard, where exponential growth takes off.

33rd Square explores technological progress in AI, robotics, genomics, neuroscience, nanotechnology, art, design and the future as humanity encroaches on The Singularity.











Copyright 2012-2014 33rd Square | Privacy Policy | RSS | News | Submit an Article | Link to Us | Store | About Us | Contact Us